Тринатрийтиофосфат

Description

Trisodium thiophosphate is an inorganic salt of monothiophosphoric acid, a structural analogue of trisodium phosphate, in which one oxygen atom of the phosphate group is replaced by a sulfur atom. Formula Na3PO3S, CAS 10101-88-9. In chemical and biochemical practice, the compound is used as a reagent for the preparation of phosphorothioate derivatives of nucleotides and oligonucleotides.

Basic Attributes

• CAS No.: 10101-88-9
• Chemical formula: Na3PO3S (H3O3PS, 3Na)
• Molecular weight: 180.01
• Exact weight: 179.90000
• EU number: 233-261-6
• UNII: AN1017219I
• PSA Specifications: 101
• XLogP3: 1.15300

Synonyms

Phosphorthionic acid, sodium salt; phosphorothioic acid, trisodium salt; sodium phosphorothioate (Na3PO3S); trisodium phosphorothioate; trisodium monothiophosphate; sodium thiophosphate.

Physico-chemical characteristics

• Density: 2.149 g/cm3
• Melting point: 56–58 °C

Chemical properties

Like other thiophosphates, Na3PO3S exhibits more pronounced nucleophilic and reducing properties compared to ordinary sodium orthophosphate - the sulfur atom is more easily polarized and more readily participates in alkylation reactions than the oxygen atom. This property underlies the use of salt to introduce a phosphorothioate bond (P=S) into the chains of synthetic nucleotides instead of the standard phosphodiester bond (P=O).

Scope of application

Sodium monothiophosphate is used as a laboratory reagent in biochemistry and molecular biology. The main direction is the synthesis of phosphorothioate analogues of nucleotides and oligonucleotides for RNA interference (RNAi) studies, including work on light-activated dsRNA precursors and photoelectrochemical detection of nucleic acids by enzymatic hybridization. Also appears as a general-purpose biochemical reagent for proteomic studies.

Safety Information

• Hazard label: Xi (irritant)

Data on UN hazard category, transport codes, GHS H- and P-phrases and RTECS number are not available in the source.

Storage and transportation

Store in a dry, well-ventilated area, in tightly closed original containers, away from acids and strong oxidizing agents. When working with a substance marked Xi, use gloves and safety glasses, avoid the formation of dust and contact with skin and mucous membranes.

Place in the series of sodium thiophosphates

Sodium monothiophosphate Na3PO3S is the simplest representative of the series of thiosodium phosphates, which also includes dithio- and trithiophosphates. In terms of solubility and alkalinity of the aqueous solution, the compound is close to trisodium phosphate, but is characterized by reduced resistance to hydrolysis and oxidation - in air and in the presence of moisture, sulfur gradually oxidizes to sulfate, so the reagent is stored in a sealed container without access to oxygen and excess moisture, preferably at a low temperature.