Фотобиотин

Description

Photobiotin is a photoactivatable biotinylation reagent for non-radioactive labeling of nucleic acids. The molecule consists of three parts: a biotin group, a linker and a photoactivatable aryl azide group bearing nitro and azido substituents on the benzene ring. When irradiated with strong visible light for 15–20 minutes, the aryl azide is converted to the highly reactive arylnitrene, which covalently binds to single- and double-stranded DNA, RNA, and proteins. The resulting biotinylated probes detect up to 0.5 pg of target DNA upon hybridization.

Basic Attributes

• CAS No.: 96087-37-5
• Chemical formula: C23H35N9O4S
• Molecular Weight: 533.64700
• Exact weight: 533.25327181
• PSA Specifications: 206.37000
• XLogP3: 4.59996

Physico-chemical characteristics

• Melting point: 114.5–115 °C
• Appearance: Orange to red powder

Scope of application

• preparation of biotinylated DNA and RNA probes for hybridization without a radioactive label
• nonspecific labeling of proteins and other biomolecules with biotin
• affinity methods based on the biotin-streptavidin pair
• molecular biological diagnostics, blot hybridization, in situ hybridization

Operating procedure

The mixture of nucleic acid and photobiotin is exposed to strong visible light for 15–20 minutes. The aryl azide group is converted to arylnitrene and forms a covalent bond with the target. The reagent is photosensitive for its intended purpose, so all operations until activation are carried out under protected lighting.

Safety Information

• WGK Germany: 3

Storage and handling

Storage conditions according to the card: −20 °C. Store in the dark, in a tightly closed container, protect from premature exposure. Class WGK Germany 3 indicates a strong danger to the aquatic environment: do not dispose of solutions or washes down the drain; hand over the waste to a licensed operator. Wear gloves and safety glasses.