Амберлит XAD 2

Description

Amberlite XAD 2 is a nonionic macroporous polymer sorbent based on a cross-linked copolymer of styrene and divinylbenzene, the closest analogue of Amberlite XAD 4 with a slightly larger pore size. Like other representatives of the XAD series, it does not contain ion-exchange functional groups and retains organic substances due to physical adsorption on the developed internal surface of the hydrophobic polymer matrix, and not due to ion exchange. A larger pore structure compared to XAD 4 makes the resin suitable for the sorption of larger organic molecules - dyes, humic substances, individual proteins and biomolecules of moderate molecular weight. The choice between XAD 2 and XAD 4 in practice is determined by the size of the target analyte: for low-molecular phenols and pesticides, fine-porous XAD 4 with a more developed specific surface area is preferable, while for large molecules - dyes, polyphenolic complexes, peptides - large-porous XAD 2 is more effective, since the size of its pores does not create diffusion restrictions for the penetration of bulk molecules into the depth of the granule.

Basic Attributes

• CAS No.: 2615261 (sources also include 37339-42-7)
• Exact weight: 414.267120
• PSA specifications: 15.71000
• XLogP3: 5.14

Physico-chemical characteristics

• Density: 1.1 ± 0.1 g/cm³
• Boiling point: 540.6 ± 50.0 °C at 760 mm Hg. Art.
• Flash point: 143.9 ± 27.3 °C
• Refractive index: 1.577

Scope of application

Solid-phase extraction and concentration of medium-polar and large organic molecules from aqueous media: purification and separation of natural pigments, humic acids and tannins, extraction of phenolic compounds and aromatic pollutants in the analysis of natural waters, preparative separation of alkaloids and other plant metabolites, as well as use in biochemistry as a non-specific adsorbent for removing hydrophobic impurities from protein solutions. Compared to XAD 4, the larger pore size reduces the retention efficiency of low molecular weight analytes but increases accessibility for bulky molecules. Both resins of the XAD series complement each other in analytical and preparative practice: the sequential use of cartridges with sorbents of different porosities allows the fractionation of complex mixtures of natural compounds by molecular size even at the stage of sample preparation, before chromatographic separation.

Storage and handling

Store in a dry place, in original containers, away from sources of contamination. Detailed data on the classification of hydrochloric acid and transport codes for resins are not published in open sources, which is typical for inert polymer sorbents. When working with dry powder, use gloves and safety glasses, avoid inhaling dust; Before first use, wash the resin with methanol to remove residual monomers and low molecular weight impurities formed during polymerization of the copolymer.