ХЛОР (ДИИЗОПРОПИЛАМИНО) МЕТОКСИФОСФИН

Identification

Chloro(diisopropylamino)methoxyphosphine is a phosphoramidochloridite, a reagent from the class of chlorophosphites with one amino group and one methoxy group at the phosphorus atom. CAS number 86030-43-5, formula C7H17ClNOP, English name Chloro(diisopropylamino)methoxyphosphine. Synonyms: N,N-diisopropylmethylphosphonamide chloride; methyl N,N-diisopropylchlorophosphoramidite; methyl N,N-diisopropylphosphoramidochloridite; N,N-methyl ester of diisopropylamidochloridephosphoric acid.

Basic Attributes

• Molecular weight: 197.64
• Exact weight: 197.07400
• HS code: 2929909090
• PSA: 26.06000
• XLogP3: 3.21730

Physico-chemical characteristics

• Appearance: cloudy, viscous liquid
• Density: 1.023 g/ml at 20 °C (lit.)
• Boiling point: 34-35 °C at 0.8 mm Hg. Art.
• Flash point: 163°F (72.8°C)
• Refractive index: n20/D 1.470
• Storage conditions: refrigerator

Substance class and purpose

The reagent belongs to phosphoramidochloridites - compounds of trivalent phosphorus with one chloride, one amino group (diisopropylamino) and one alkoxy group (methoxy). Such phosphoramidite reagents are used to obtain phosphoramidite building blocks in the synthesis of oligonucleotides (DNA and RNA) by solid-phase phosphoramidite synthesis: the chlorine atom is replaced by the alcohol group of the 5'-protected nucleoside, yielding the nucleoside phosphoramidite, which is then used in the automatic chain extension cycle. The diisopropylamine group provides the desired resistance to premature hydrolysis while maintaining the reactivity of the chloride. In the standard cycle of oligonucleotide synthesis, the phosphoramidite obtained from this reagent is activated by tetrazole or its derivatives, after which it combines with the free 5'-hydroxy group of the growing chain, and the resulting phosphite is oxidized to phosphate at the next stage of the cycle.

Safety Information

• Packing level: III
• Hazard category: 8
• Transport codes: UN 3265, class 8, packing group II
• WGK Germany: 3
• Hazard class code: 34
• Safety instructions: 26-36/37/39-45
• Tags: C
• Hazard Statements: H314
• Precautions: P280-P305+P351+P338-P310

Storage and transportation conditions

Store in the refrigerator, in an airtight container under an inert atmosphere, isolated from moisture - phosphoramidochloridite hydrolyzes with water, releasing hydrogen chloride. The substance is corrosive (H314), when working, gloves, safety glasses and a fume hood are required, avoid inhalation of vapors and contact with skin. Transported as dangerous goods of class 8, packing group II-III, UN number 3265. After use, do not mix the container with water-containing waste without first extinguishing the reagent residues with anhydrous isopropanol alcohol in a fume hood.