Динатрийкарбамилфосфат

Description

Disodium carbamyl phosphate is the disodium salt of carbamyl phosphoric acid (mixed anhydride of carbamic and phosphoric acids), a key biochemical intermediate of nitrogen metabolism. English name Disodium carbamyl phosphate, CAS 72461-86-0, chemical formula CH4NO5P,2Na. The substance belongs to the class of phosphines and phosphorylated biochemical reagents.

Basic Attributes

• CAS No.: 72461-86-0
• Chemical formula: CH4NO5P,2Na
• Molecular Weight: 184.98
• Exact weight: 184.947
• EU number: 276-671-0
• PSA specification: 116
• XLogP3: 0.7511

Physico-chemical characteristics

• Boiling point: 403.3 °C at 760 mm Hg. Art.
• Flash point: 197.7 °C
• Storage conditions: -20 °C

Scope of application

Carbamyl phosphate is a natural substrate of carbamoyltransferase enzymes involved in the urea cycle (synthesis of citrulline from ornithine) and in the biosynthesis of pyrimidine nucleotides. Disodium salt is used as a biochemical reagent in research laboratories to study enzymatic reactions of carbamyl group transfer, as a substrate when working with ornithine transcarbamylase and carbamoyl phosphate synthetase, and in analytical biochemistry for the calibration of enzyme tests.

Safety Information

• Transport codes: NONH for all modes of transport
• WGK Germany: 3

Other information about the GHS classification, RTECS number and hazard statements is not provided in the manufacturer's original data.

Storage and transportation conditions

Store at -20°C - the compound is unstable at room temperature and is prone to hydrolysis of the carbamyl phosphate bond over time, so the reagent is supplied and stored frozen in an airtight container, protected from moisture. Transportation is carried out as for a laboratory biochemical reagent not classified as dangerous goods (code NONH), in compliance with the cold chain.

Place in the biochemistry of nitrogen metabolism

In cells, carbamoyl phosphate is synthesized by carbamoyl phosphate synthetase from ammonia, bicarbonate and ATP and serves as a donor of the carbamyl group in two ways: the urea cycle, which neutralizes ammonia in mammals, and the biosynthesis of pyrimidine bases necessary for the synthesis of RNA and DNA. This dual role makes this compound a classic target for studies of the regulation of nitrogen metabolism in biochemical and medical laboratories, including studies of inherited deficiencies of urea cycle enzymes.

Working with a reagent in the laboratory

Due to the thermodynamic instability of the carbamyl phosphate bond, reagent solutions are prepared immediately before the experiment and used for a limited time, maintaining a low temperature of the working solution. The exact concentration of the substrate in an enzymatic assay is important for quantitative assessment of the activity of carbamoyltransferases, therefore the reagent is packaged and stored in small portions, eliminating repeated freezing-thawing of the batch.